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Phenylmethanesulfonyl fluoride ?98.5% (GC) | Sigma-Aldrich
Synonym: ?-Toluenesulfonyl fluoride Benzylsulfonyl fluoride PMSF Phenylmethylsulfonyl fluoride. CAS Number . P8340Protease Inhibitor Cocktail. for use with .
http://www.sigmaaldrich.com/catalog/product/sigma/p7626?lang=en®ion=US

Broad Spectrum Inhibitors - Sigma-Aldrich
P7626, PMSF, Broad spectrum serine protease inhibitor. Also reported to inhibit some cysteine proteases such as .
http://www.sigmaaldrich.com/life-science/metabolomics/enzyme-explorer/learning-center/protease-inhibitors/broad-spectrum-inhib.html




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Protease Inhibition and Detection - Sigma-Aldrich
stable 6 months at -20 C. P7626. PMSF. Broad spectrum serine protease inhibitor. Also reported to inhibit some cysteine proteases such as papain. 0.1-1.0 mM .
http://www.sigmaaldrich.com/etc/medialib/flashapps/biofiles-movie/pdf/biofiles-issue42.Par.0001.File.dat/BioFiles_4.2_v1.pdf

Phenylmethanesulfonyl fluoride (P7626) - Product ... - Sigma-Aldrich
Melting Point: 91-92C1 Sigma typically finds values in the range of 91-94C. PMSF is a inhibitor of serine proteases and acetylcholinesterase.2 It inhibits .
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/1/p7626pis.Par.0001.File.tmp/p7626pis.pdf


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When to use PMSF in a cell lysis buffer? - General Lab Techniques
Oct 14, 2008 . I usually use Sigma P2714, a protease inhibitor mixture, for general use. I noticed that many times, people included PMSF in lysis buffer.
http://www.protocol-online.org/biology-forums/posts/40345.html

PMSF | Protocols Online
Apr 11, 2010 . PMSF is commonly used in protein solublization in order to deactivate . PMSF does not inhibit all serine proteases. . PMSF: Sigma (P-7626) .
http://protocolsonline.com/recipes/pmsf-10-mm-10-ml/


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Cell Extraction Protocol | Life Technologies
1 mM PMSF Protease Inhibitor Cocktail, Sigma (Cat. no. P-2714) This Cell Extraction Buffer must be supplemented with 1 mM PMSF (not included) and Protease .
http://www.invitrogen.com/site/us/en/home/References/protocols/cell-and-tissue-analysis/elisa-protocol/ELISA-Sample-Preparation-Protocols/Cell-Extraction-Protocol.html


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PR-619, A Novel small molecule reversible inhibitor of ...
1mM PMSF, protease inhibitor cocktail (Sigma, Cat. No. P-. 8849) and 20g/ml aprotinin). Total cell lysates (25g) were boiled in the presence of 1X Laemmli .
http://www.lifesensors.com/pdf/PR-619_application_note.pdf


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Small Scale Nuclear and Cytoplasmic Extracts for Neurons ...
+ protease inhibitors ( aprotinin. benzamidine, leupeptin, pepstatin, PMSF, or PMSF and. 100x Protease inhibitor cocktail from Calbiochem, we use 'Protease .
http://graeflab.stanford.edu/pdf/Neuron_Nuclear_Extract_.pdf


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Activation of nuclear factor ?B in colonic mucosa from patients with ...
Jun 7, 2004 . Phosphorylation targets the inhibitor to polyubiquitination and proteasomal . fluoride (PMSF), and protease inhibitor cocktail (#P8340; Sigma, .
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1774469/


Cell Death and Differentiation - Figure 1 for article: The post ...
. 70 mM DTT and 2% Ampholyte pH 8.5?10.5 (Sigma) containing 1 mM PMSF, Protease Inhibitor Cocktail (Sigma) and Phosphatase Inhibitor Cocktail I (Sigma) .
http://www.nature.com/cdd/journal/v12/n2/fig_tab/4401526f1.html

TALO Purification
100x Protease inhibitors: 100mM PMSF, 200M pepstatin, 60M leupeptin, . 1000x Histone deacetylase inhibitors: 500M Trichostatin A (Sigma), 25mM .
http://labs.fhcrc.org/tsukiyama/protocols/TALO8_Protocol.pdf

Whole Cell Lysis of Yeast for SDS-PAGE BIO-PROTOCOL
100% (w/v) Trichloroacetic acid (TCA) (Sigma). 4. Pepstatin . (Protease inhibitors: pepstatin, PMSF, leupeptin, and DTT, all in 1000x stock solution). ( Pipette up .
http://www.bio-protocol.org/wenzhang.aspx?id=14

PMSF (phenylmethylsulfonyl fluoride)
Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain.
http://www.piercenet.com/browse.cfm?fldID=02040802

ChIPs screening protocol
Resuspend tissue in 10 mL cold PBS (plus protease inhibitors) per gram of starting material. Put on . 100 mM PMSF (Sigma P-7626) in ethanol, use at 1: 100 .
http://farnham.genomecenter.ucdavis.edu/protocols/tissues.html


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How to Prepare a Protease Inhibitor Cocktail | eHow.com
Protease inhibitors are enzymes that inhibit the actions of proteases, which are certain enzymes that kill proteins . Make PMSF (Phenylmethyl sulfonyl fluoride) at stock 100mM. . Protease Inhibitors Cocktail · Sigma: List of protease inhibitors .
http://www.ehow.com/how_7158843_prepare-protease-inhibitor-cocktail.html

N.1
500 ml PMSF (Sigma #P7626). 100 ml pepstatin (2.0 mg/ml). 100 ml leupeptin ( 1.0 mg/ml). 100 ml aprotinin (5.0 mg/ml). (Protease Inhibitor Cocktail Sigma .
http://genetics.med.harvard.edu/~cepko/protocol/mike/N1.html


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PMSF - Wikipedia, the free encyclopedia
In biochemistry, PMSF (phenylmethanesulfonylfluoride or phenylmethylsulfonyl fluoride) is a serine protease inhibitor commonly used in the preparation of cell .
http://en.wikipedia.org/wiki/PMSF


Griffin: Ultimate Immunoprecipitation Guide - OpenWetWare
Apr 17, 2012 . PMSF : Stock Solution 100 mM (100x stock) or 200 mM in . S8820: Sigma SIGMAFAST Protease Inhibitor Tablets; 78429: PIERCE Halt .
http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide


Biochemical Journal
zyme, 20 g/ml PMSF, protease inhibitor cocktail (Sigma, Castle. Hill, NSW, Australia) (1:1000), 25 g/ml DNase and 5 mM 2- mercaptoethanol were added.
http://www.biochemj.org/bj/405/0489/4050489.pdf


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Nuclear Extraction Kit Protocol
P5726). Protease Inhibitor cocktail (Sigma Catalogue no. P8340). 0.1 M DTT . 100 mM PMSF (Sigma P7626) in 100% ethanol (store @ -20C). Cold Phosphate .
http://www.diagnostictechnology.com.au/persistent/catalogue_files/products/Marl-NE-p.pdf

Chapter 2 Materials and Methods
1% protease inhibitor cocktail (Sigma). Extraction buffer (for nuclear . 50 g/ml PMSF (Sigma). 1 g/ml leupeptin . proteinase K (Invitrogen). RNase A (ICN .
ftp://ftp.sanger.ac.uk/pub4/theses/jim/chapter2.pdf

Human OTU domain containing 5, OTU domain - SGC | Structure ...
Human Otud5 or DUBA is a deubiquitinating enzyme which .
http://www.thesgc.org/structures/details?pdbid=3PFY/

Cdx2 regulates endo-lysosomal function and epithelial cell polarity
Leupeptin serves as a nonspecific protease inhibitor. ?-Actin serves as a loading . 1 mM PMSF, and protease inhibitors (Sigma), from E18.5 mouse intestines.
http://genesdev.cshlp.org/content/24/12/1295.long

 

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GUIDE FOR LAB METHODS AND REAGENTS FOLDER
PMSF 1 mM; 100x stock solution:100 mM in 2-Propanol, store at RT. ? Protease inhibitor cocktail (CLAP): [Sigma P8340] DMSO solution, store @ -20C, use as .
http://www-p53.iarc.fr/FP6material/GST%20pull-down_CIB.doc

GST Fusion Protein Production
(a) Proteinase Inhibitor cocktail. 1 tablet per 7ml buffer. (b) PMSF. 1mM . (a) Weigh out the required quantity of glutathione agarose (Sigma cat# 4510). From a .
http://www.oardc.ohio-state.edu/stockingerlab/Protocols/FusionProteinProduction(NoCBF).pdf


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The PARP Link : PARP purification
3-Aminobenzamide (3-AB), 99%, (Sigma-Aldrich) 1 g (#A-0788) . Proteases inhibitor cocktail tablets (Complete Mini, Roche Diagnostic Gmbh, Mannheim, Germany) . pH 7.5, 14 mM beta-Mercaptoethanol, 0.5 mM EDTA and 0.5 mM PMSF.
http://parplink.u-strasbg.fr/protocols/tools/parp_purification.html

Supplemetary Methods Recombinant human XIAP (BIR2 domain ...
Add protease inhibitors to the Extraction Buffer immediately prior to use. . 2 g/ mL Leupeptin, 2 g/mL Antipain, 2 g/mL Pepstatin, 100 M PMSF, and 1 mM DTT. . Bovine heart (Sigma, Catalog # C3131), 2 mg/mL stock in deionized water .
http://www.rndsystems.com/literature_human_xiap_bir2.aspx


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